Recombinant:Article Title: HCAR1/MCT1 Regulates Tumor Ferroptosis through the Lactate-Mediated AMPK-SCD1 Activity and Its Therapeutic Implications.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HCAR1 abcam Abcam Cat# ab124010, RRID:AB_10976142 GPX4 abcam Abcam Cat# ab125066, RRID:AB_10973901 ACSL4 abcam Abcam Cat# ab155282, RRID:AB_2714020 SCD1 CST Cell Signaling Technology Cat# 2794, RRID:AB_2183099) SLC7A11 CST Cell Signaling Technology Cat# 12691, RRID:AB_2687474 pAMPK CST Cell Signaling Technology Cat# 2535, RRID:AB_331250 AMPK CST Cell Signaling Technology Cat# 5832, RRID:AB_10624867 MCT1 Proteintech Cat# 20139-1-AP SREBP1 Proteintech Proteintech Cat# 14088-1-AP, RRID:AB_2255217 FSP1 Proteintech Cat# 20886-1-AP a-SMA Proteintech Proteintech Cat# 14395-1-AP, RRID:AB_2223009 Vimentin Proteintech Proteintech Cat# 10366-1-AP, RRID:AB_2273020 b-actin Bioss Bioss Cat# bs-0061R, RRID:AB_10855480 Biological Samples Paraffin liver cancer sections Chongqing University Cancer Hospital N/A Fresh liver biopsy or surgically resected samples Chongqing University Cancer Hospital N/A Chemicals, Peptides, and Recombinant Proteins QuickBlock Beyotime Cat# P0260 Biotin-labeled Goat Anti-Rabbit Beyotime Cat# A0279 HRP-labeled Goat Anti-Rabbit Beyotime Cat# A0208 BeyoECL Moon Beyotime Cat# P0018FS PVDF Millipore Cat# ISEQ00010 BamHI NEB Cat# R0136 EcoRI NEB Cat# R3101 pLVshRNA-Puro Inovogen Cat# VL3103 pLV-IRES-Puro Inovogen Cat# VL3002 siHCAR1 RIBOBIO Cat# SIGS0000115-1 siMCT1 RIBOBIO Cat# siB1516103136-1-5 L-lactate Sigma Cat# L1750 pyruvate Sigma Cat# 107360 sodium lactate Sigma Cat# L7022 BODIPY C11 Thermo Fisher Cat# D3861 Lipofectamine Thermo Fisher Cat# L3000015 AZD3965 MCE Cat# HY-12750 RSL3 MCE Cat# HY-100218A Liproxstatin-1 MCE Cat# HY-12726 Collagenase IV Thermo Fisher Cat# 17104019 Dispase II Sigma Cat# 4942078001 Critical Commercial Assays Annexin V-FITC/PI BD biosciences Cat# 556570 Improved Citrate Antigen Retrieval Solution Beyotime Cat# P0083 (Continued on next page) Cell Reports 33, 108487, December 8, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER DAB Horseradish Peroxidase Color Development Kit) Beyotime Cat# P0203 Cell Counting Kit-8 Beyotime Cat# C0038 ATP assay kit Beyotime Cat# S0026B Cell lysis buffer for Western and IP Beyotime Cat# P0013 AMP-GLOTM assay kit Promega Cat# V5011 FastKing gDNA Dispelling RT SuperMix Tiangen Cat# KR118 Q-PCR kit Tiangen Cat# FP314 RNAprep pure Cell Kit Tiangen Cat# DP430 Experimental Models: Cell Lines Hep3B ATCC HB-8064 Huh-7 The Cell Bank of Type Culture Collection of Chinese Academy of Sciences TCHu182 HepG2 ATCC HB-8065 Experimental Models: Organisms/Strains BALB/c Vital River Laboratory Animal Technology Co., Ltd. https://buy.vitalriver.com/commodity/detail? productId=4028b2ea69b5248a0169b52627260647 (Identifier: 401) Oligonucleotides Primers for HCAR1, see Table S1 This paper N/A Primers for SCD1, see Table S1 This paper N/A Primers for ACSL4, see Table S1 This paper N/A Primers for GPX4, see Table S1 This paper N/A Primers for FSP1, see Table S1 This paper N/A shHCAR1 sequence 50-GCTGCTCATT GTGGCCTTTGT-30 This paper N/A Recombinant DNA pLV-shMCT1 This paper N/A pEnCMV-SCD1 This paper N/A Software and Algorithms GraphPad Prism version 8 GraphPad Software https://www.graphpad.com/scientific-software/prism/ NIS-Elements D Nikon Corporation https://www.nikon.com/products/microscope-solutions/ support/download/software/imgsfw/#accordion-local-03 CytExpert2.3 Beckman Coulter Life Sciences https://www.beckman.ae/flow-cytometry/instruments/ cytoflex/software Image Lab Software Bio-Rad https://www.bio-rad.com/en-cn/product/image-lab- software?ID=KRE6P5E8Z iMark Microplate Absorbance Reader Bio-Rad https://www.bio-rad.com/en-cn/product/ imark-microplate-absorbance-reader?
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